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# 235 by antibodys
30.10.2014 - 07:57 email IP: logged quote

Size: 25T
Length: 743bp
Accession NO.: NM_000367
Application ares: RT-PCR
Storage: The Primer Pair is stable for up to one year at
-20°C in a non-frost free freezer?
Avoid repeated freeze-thaw cycles.
Description: Human TPMT Primer Pair for RT-PCR (reverse transcription followed by polymerase chain reaction) analysis of mRNA expression.
Quality Control: Primer Pairs for RT-PCR provide convenient
primers for analyzing the expression of specific
mRNAs or got specific PCR fragment by RT-PCR. Each Primer Pair includes a 5´ and 3´ oligonucleotide primer provided in individual vials at 50?M ready for addition to the amplification reaction. The Primer Pairs have been confirmed in two-step conventional RT-PCR using human esophageal carcinoma EC109 cells total RNA.
PCR products from the use of the Primer Pairs have been sequence-confirmed for appropriate.
Technical Hints: • Thaw all reagents completely on ice before use.
• Use either random primers or oligo (dT) for reverse transcription.
• All PCR reactions should be assembled on ice.
• The recommended annealing
sx: TPMT
The information is from theunglücklich www.bioabb.com/products/Human-TPMT-Primer-Pair.html )

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